Plasma Total Amino Acids, Plasma Glutamate and Alanine Levels in Diabetic Subjects

Kashinath R.T.; nagendra subbaiah; Rudrappa. G.

Plasma Total Amino Acids, Plasma Glutamate and Alanine Levels in Diabetic Subjects

Kashinath R.T. * § Msc Biochemistry, Ph.D. Biochemistry (Medical Faculty), FICS

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nagendra subbaiah

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Rudrappa. G.

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§ Rajive Gandhi University of Health Science, Bangalore.

Plasma Total Amino Acids, Plasma Glutamate and Alanine Levels in Diabetic Subjects

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Abstract

Generally, in fasting state, gluconeogenesis accounts for about 30% of overall hepatic glucose output, the increase in type 2 diabetic subjects may be much more than this level. There is a close relationship between glucose metabolism and amino acid metabolism which is established through transamination reactions. The key amino acid -keto acid pairs involved in transamination reactions are glutamateα ketoglutarate, aspartate -oxaloacetate and pyruvate -alanine. A study was undertaken to assess the plasma levels of total amino acids, glutamate and alanine in type 2 diabetic subjects. A blood sample (5ml) with heparin as an anticoagulant was collected in the fasting state, from each of the selected normal as well as diabetic subjects. The separated plasma samples were employed for the estimation of total amino acid nitrogen levels, as well as for the estimation of plasma alanineand glutamate levels. The results indicate a significant rise in the plasma levels of total amino acids, alanine & glutamate in type-2 diabetic subjects as well as a significant increase in plasma alanine levels in diabetics of 30-40 yrs age as compared to diabetics of 41-60yrs of age. These findings suggest an increased availability of glucogenic amino acid precursors for glucose formation may be due to lack of proteolytic suppression of insulin.

References

19 Cites in Article

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Kashinath R.T., nagendra subbaiah, Rudrappa. G., Dr. R. T. Kashinath. 2014. "Plasma Total Amino Acids, Plasma Glutamate and Alanine Levels in Diabetic Subjects". Global Journal of Medical Research - K: Interdisciplinary GJMR-K Volume 14 (GJMR Volume 14 Issue K1).

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Journal Specifications

Crossref Journal DOI 10.17406/gjmr

Print ISSN 0975-5888

e-ISSN 2249-4618

Keywords

alanine amino acids gluconeogenesis glutamate

Classification

GJMR-K Classification NLMC Code: WK 830

QU 55

Submission ReceivedDecember 11, 2013
Peer Review Double Blind
Handling Editor
Accepted January 5, 2014
Published January 15, 2014

Version of record

v1.2

Issue date

March 13, 2014

Language

English

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Article Matrices

Total Score: 144

Country: India

Subject: Global Journal of Medical Research

Authors: Kashinath R.T., Nagendra .S., Rudrappa. G., Srinivas.S. (PhD/Dr. count: 0)

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