Proteomix and Enzyme Kinetics in Normal and Cataractous Human Lens

Proteomix and Enzyme Kinetics in Normal and Cataractous Human Lens

Dr. Ajit V. Pandya

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Gujarat University

Proteomix and Enzyme Kinetics in Normal and Cataractous Human Lens

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Abstract

Opacity of lens is called cataract. Blindness due to cataract increases to great extent globally, more than 50% people are experiencing profound or total loss of vision due to cataract. Oxidative damage plays major role in cataract development and defects are recorded in the antioxidant and related enzymes in lens during this disease. The mean value of GLUTATHION REDUCTASE activity is 1.463 ± 0.079 and 0.730 ± 0.062 n moles/min/mg, GLUTATHION-S-TRANSFERASE activity is 1.780 ± 0.069 and 0.545 ± 0.342 n moles/min/mg and Y-GLUTAMYL TRANSPEPTIRASE activity is 9.595 ± 0.094 and 3.7 ± 0.216 n moles/min/mg respectively for normal and cataractous lenses. An explanation for fall in GLUTATHION REDUCTASE activity would be the inhibitory effect of oxidants on the activity of reducing enzymes. The activity of GLUTATHION-S-TRANSFERASE is very low compared to GLUTATHION REDUCTASE and Y-GLUTAMYL TRANSPEPTIRASE. The turn over of GSH by Y-GLUTAMYL TRANSPEPTIRASE and GLUTATHION-S-TRANSFERASE is thought to be groups led with several factors including GSH level. Altered activity of the enzymes associated with the synthesis, catabolism and utilization of glutathione in the lens have been reported in mixed type of cataract.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Dr. Ajit V. Pandya. 2014. \u201cProteomix and Enzyme Kinetics in Normal and Cataractous Human Lens\u201d. Global Journal of Science Frontier Research - G: Bio-Tech & Genetics GJSFR-G Volume 14 (GJSFR Volume 14 Issue G2): .

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GJSFR Volume 14 Issue G2
Pg. 1- 4

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Keywords

glutamyl trans peptidase (GTP)glutathion reductase (GR)glutathione s- transferase (GST)human lens

Submission ReceivedMarch 1, 2014
Peer Review Double Blind
Handling Editor
Accepted March 27, 2014
Published April 8, 2014

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July 24, 2014

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Total Score: 106

Country: India

Subject: Global Journal of Science Frontier Research - G: Bio-Tech & Genetics

Authors: Dr. Ajit V. Pandya (PhD/Dr. count: 1)

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