RSM for Accelerated Biofilm Formation That Facilitates Bioremediation and Characterization of Biofilm

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Dr. R. Vidhyalakshmi
Dr. R. Vidhyalakshmi
2
C. Vallinachiyar
C. Vallinachiyar
1 Sathyabama university

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GJSFR Volume 12 Issue G5

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Pseudomonas stutzeuri a sewage isolate has been reported to produce extracellular polymeric substances(EPS) invitro and forms high quality biofilm in natural environment .An attempt is made to increase the extracellular polymeric substances and its Biofilm forming ability of the isolate using Design Expert-8 a statistical software .Various factors like concentration of carbon source, Nitrogen source , pH, Temperature are known to influence the process of EPS synthesis. FTIR reports strongly infer the presence of anionic glycoprotein polymer with stretching-NH vibrations around 3100 -3500nm and -CH vibration around 1637.99 indicating the presence of aldehyde group. HPLC further ensured the presence of glucose ,galactose ,rhamnose and verbacose at a concentration of 30%,22%,26%,25%and 5% respectively. Phenol sulphuric acid assay and Barfoards assay showed the presence of 60% and 30% of carbohydrate and protein components respectively. The polymer showed metal binding ability of 26% and 42% of lead and copper respectively complimented by its anionic nature. Extracted EPS showed noticeable emulsifying effect on diesel, engine oil, petrol and grease that can route this to successful tool for bioremediation.

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No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

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No ethics committee approval was required for this article type.

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Not applicable for this article.

Dr. R. Vidhyalakshmi. 2012. \u201cRSM for Accelerated Biofilm Formation That Facilitates Bioremediation and Characterization of Biofilm\u201d. Global Journal of Science Frontier Research - G: Bio-Tech & Genetics GJSFR-G Volume 12 (GJSFR Volume 12 Issue G5): .

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GJSFR Volume 12 Issue G5
Pg. 13- 17
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Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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v1.2

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October 2, 2012

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English

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Pseudomonas stutzeuri a sewage isolate has been reported to produce extracellular polymeric substances(EPS) invitro and forms high quality biofilm in natural environment .An attempt is made to increase the extracellular polymeric substances and its Biofilm forming ability of the isolate using Design Expert-8 a statistical software .Various factors like concentration of carbon source, Nitrogen source , pH, Temperature are known to influence the process of EPS synthesis. FTIR reports strongly infer the presence of anionic glycoprotein polymer with stretching-NH vibrations around 3100 -3500nm and -CH vibration around 1637.99 indicating the presence of aldehyde group. HPLC further ensured the presence of glucose ,galactose ,rhamnose and verbacose at a concentration of 30%,22%,26%,25%and 5% respectively. Phenol sulphuric acid assay and Barfoards assay showed the presence of 60% and 30% of carbohydrate and protein components respectively. The polymer showed metal binding ability of 26% and 42% of lead and copper respectively complimented by its anionic nature. Extracted EPS showed noticeable emulsifying effect on diesel, engine oil, petrol and grease that can route this to successful tool for bioremediation.

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RSM for Accelerated Biofilm Formation That Facilitates Bioremediation and Characterization of Biofilm

Dr. R. Vidhyalakshmi
Dr. R. Vidhyalakshmi Sathyabama university
C. Vallinachiyar
C. Vallinachiyar

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