RSM for Accelerated Biofilm Formation That Facilitates Bioremediation and Characterization of Biofilm

RSM for Accelerated Biofilm Formation That Facilitates Bioremediation and Characterization of Biofilm

Dr. R. Vidhyalakshmi

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C. Vallinachiyar

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Sathyabama Institute of Science and Technology

RSM for Accelerated Biofilm Formation That Facilitates Bioremediation and Characterization of Biofilm

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Abstract

Pseudomonas stutzeuri a sewage isolate has been reported to produce extracellular polymeric substances(EPS) invitro and forms high quality biofilm in natural environment .An attempt is made to increase the extracellular polymeric substances and its Biofilm forming ability of the isolate using Design Expert-8 a statistical software .Various factors like concentration of carbon source, Nitrogen source , pH, Temperature are known to influence the process of EPS synthesis. FTIR reports strongly infer the presence of anionic glycoprotein polymer with stretching-NH vibrations around 3100 -3500nm and -CH vibration around 1637.99 indicating the presence of aldehyde group. HPLC further ensured the presence of glucose ,galactose ,rhamnose and verbacose at a concentration of 30%,22%,26%,25%and 5% respectively. Phenol sulphuric acid assay and Barfoards assay showed the presence of 60% and 30% of carbohydrate and protein components respectively. The polymer showed metal binding ability of 26% and 42% of lead and copper respectively complimented by its anionic nature. Extracted EPS showed noticeable emulsifying effect on diesel, engine oil, petrol and grease that can route this to successful tool for bioremediation.

References

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Dr. R. Vidhyalakshmi. 2012. \u201cRSM for Accelerated Biofilm Formation That Facilitates Bioremediation and Characterization of Biofilm\u201d. Global Journal of Science Frontier Research - G: Bio-Tech & Genetics GJSFR-G Volume 12 (GJSFR Volume 12 Issue G5).

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GJSFR Volume 12 Issue G5

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Keywords

Anionic polymer Biofilms Extracellular polymeric substances RSM

Submission ReceivedAugust 2, 2012
Peer Review Double Blind
Handling Editor
Accepted August 30, 2012
Published September 14, 2012

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v1.2

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October 2, 2012

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Article Matrices

Total Score: 107

Country: India

Subject: Global Journal of Science Frontier Research - G: Bio-Tech & Genetics

Authors: Dr. R. Vidhyalakshmi, C. Vallinachiyar (PhD/Dr. count: 1)

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