Storage Response of Neem (Azadirachta Indica A. Juss.) Seed under Different Moisture and Temperature Regime

Devendra Kumar

Storage Response of Neem (Azadirachta Indica A. Juss.) Seed under Different Moisture and Temperature Regime

Devendra Kumar * §

Contact

§ Forest Research Institute

Storage Response of Neem (Azadirachta Indica A. Juss.) Seed under Different Moisture and Temperature Regime

Article Fingerprint

ReserarchID

VS1X8

Storage Response of Neem (Azadirachta Indica A. Juss.) Seed under Different Moisture and Temperature Regime Banner

Key Research Insights

Synthesized scholarly intelligence & interactive research assistant

This converted HTML version may contain rendering inconsistencies. Please refer to the PDF for the authoritative version, or click here to provide feedback.

Abstract

Effect of four storage temperatures (T1-ambient room temperature (35±5ºC), T2-Air conditioner (25±2ºC), T3- Fridge (5±1ºC) and T4-Freezer (-4±1ºC) and four moisture levels M1 (38%), M2 (21%), M3 (12%), M4 (5.5%) were studied on seed longevity of seeds under controlled conditions. After setting of desired moisture content seeds were then subjected to storage treatments in 200µ thick polythene bag and kept in airtight plastic container at different temperature levels and tested upto 270 days respectively. Seeds were sown in between paper (BP) for germination study in laboratory at 30±1ºC and relative humidity (90±3%). Freshly collected seeds after drying at target levels showed germination from 93 to 96.5 %. Seeds having M1, M2 and M3 moisture content completely lost germination well before 90 days at T1 temperature. Seeds of M4 moisture level showed 55.37% decline in germination upto 90 days. Seeds containing moisture M1, M2, M3 and M4 at T2 and T3 level showed 100%, 56.5%, 14.74%, 5.21% and 100%, 18.14%, 11.58%, 6.25% respectively decline in germination upto 90 days of storage. Seeds (M4) stored at T4 level showed 20.83% reduction in seed germination. After 150 days of seed storage, the rate of decline was observed higher in T1 at all level of moistures, T2 at M1 (100%), M2 (64.29%), M3 (8.64%), M4 (5.69%) and in T3 at M1 (100%), M2 (8.61%), M3 (16.67%), M4 (8.89%). Rate of decline in germination percentage was lower at T4 temperature and M4 (3.46%) moisture level between these two periods. Seeds stored at low temperature and moisture content (T3M4) exhibited greater mean viability period (P50, 326 days) as compared to the seeds stored at higher temperature and moisture. However, P50 was not increased when seeds were stored at lower temperature and moisture content (T4M4, 184 days). Thus present finding reveals that seeds of neem may be stored at 5±1ºC (T3) temperature with moisture 5.5% (M4) for 326 days (P50) and data also indicate the orthodox behaviour of neem seed of this region.

References

50 Cites in Article

Reference Format

1. R. Bellefontaine,M. Audinet (1993). La conservation de graines de neem (Azadirachta indica A Juss.) In: Some, L.M and De Kam, M. Tree seed problems, with special reference to Africa, 268-274.
2. P. Berjak,D. Dumet (1996). Cryopreservation of seeds and isolated embryonic axes of Neem (Azadirachta indica). Cryo-Letters, 17, 99-104.
3. P. Berjak,G. Campbell,J. Farrant,W. Omandi- Oloo,N. Pammenter (1995). Responses of seeds of Azadirachta indica (neem) to short-term storage under ambient or chilled conditions. Seed Science and Technology, 23, 779-792.
4. K. Chaisurisri,B. Ponoy,P. Wasuwanich (1986). Storage of Azadirachta indica A. Juss. Seeds. The Embryon, 1, 19-27.
5. W. Chaney,D. Knudson (1988). Germination of seeds of Azadirachta indica enhanced by endocarp removal. International Tree Crop Journal, 5, 230-233.
6. H. Chin,E. Roberts (1980). Recalcitrant crop seeds.
7. H. Chin,B. Krisinapillay,P. Stanwood (1989). Seed moisture recalcitrant vs. orthodox seeds. Seed Moisture, 5-22.
8. F. Corbineau,D. Come (1988). Storage of recalcitrant seeds of four tropical species. Seed Science and Technology, 16, 97-103.
9. J. Crowe,F. Hoekstra,I. Crowe,T. Anchordologuy,E. Dorbnis (1989). Lipid phase transitions measured in intact cells with Fourier transform infrared spectroscopy. Cryobiology, 26(1), 76-84.
CrossRef DOI
10. J. Dickie,R. Smith (1992). Limits to the survival of essentially orthodox seeds at low moisture contents in some woody species.
11. K. Djavanshir,H. Pourbeik (1976). Germination value –a new formula. Silvae Genetica, 25, 79-83.
12. R. Ellis,T. Hong,E. Roberts (1990). 1990a An intermediate category of seed storage behaviour of Coffee. Journal of Experimental Botany, 41(9), 1167-1174.
CrossRef DOI
13. R. Ellis,T. Hong,E. Roberts (1990). Moisture Content and the Longevity of Seeds of Phaseolus vulgaris. Annals of Botany, 66(3), 341-348.
CrossRef DOI
14. R. Ellis,T. Hong,E. Roberts (1991). An intermediate category of seed storage behaviour II. Effects of provenance, immaturity and imbibition on desiccation-tolerance in coffee. Journal of Experimental Botany, 42(5), 653-657.
CrossRef DOI
15. B. Ezumah (1986). Germination of storage of neem (Azadirachta indica A. Juss.). Seed Science and Technology, 14, 593-600.
16. D. Finney (1952). Plant Analysis.
17. C. Gamene,H. Kraak,J. Pijlen (1994). Azadirachta indica seeds from Burkina Faso: intermediate storage behaviour. Proceeding of the International Workshop on Desiccation Tolerance and Sensitivity of Seeds and Vegetative Plant Tissues, 23.
18. C. Gamene,H. Kraak,J. Van Pijlen,C. De Vos (1996). Storage behaviour of neem (A. indica) seeds from Burkino Faso. Seed Science and Technology, 24, 441-448.
19. D. Graham,B. Patterson (1982). Responses of plants to low, non-freezing temperatures: proteins, metabolism and acclimation. Annual Review of Plant Physiology, 33(1), 347-372.
CrossRef DOI
20. J. Hanson (1984). The storage of seeds of tropical fruits.
21. (1993). International Rules for Seed Testing. Seed Science and Technology, 21
22. M. King,E. Roberts (1979). The storage of recalcitrant seedachievements and possible approaches. International Board for Plant Genetic Resources
23. H. Kraak (1993). Physiological aspects of storage of recalcitrant seeds. Tree seed problems, with special reference to Africa, 239-253.
24. Devendra Kumar,D. Mishra,B. Singh,V. Singh (2008). Effect of methods of drying on viability, storability and seedling performance of Neem (Azadirachta indica A. Juss.) seeds. The Indian Forrester, 134, 991-1002.
25. Devendra Kumar,D. Mishra,B. Singh (2007). Effect of temperature, media and light on germination of Neem (Azadirachta indica A. Juss.) seeds. The Indian Forrester, 133, 1636-1642.
26. Devendra Kumar,D. Mishra,R. Singh (2007). Effect of different methods of seed extraction on viability, storability and seedling performance of neem (Azadirachta indica A. Juss.) seeds. My Forest, 43, 129-138.
27. Devendra Kumar,D. Mishra,S. Sharma (2009). A simplified package of seed processing of important agro forestry species (Azadirachta indica A. Juss.) for forestry field personals. Proceeding of "IV National Forestry Conference, 82-90.
28. Kumar Devendra,D. Mishra (2007). Effect of methods of seed collection on seed qualities and storability of Azadirachta indica A. Juss. (Neem) seed. Journal of Non Timber Forest Produce, 14, 271-276.
29. Kumar Devendra,D. Mishra (2007). Effect of fruits colour on germination, storability, and seedling performance in Neem (Azadirachta indica A. Juss.) seed. Journal of Non Timber Forest Produce, 14, 209-215.
30. J. Lyons,J. Raison,S. Steponkus (1979). The plant membrane in response to low temperature: an overview. Low temperature stress on crop plants. The role of the membrane, 1-24.
31. G. Maithani,V. Bahuguna,M. Rawat,O. Sood (1989). Fruit maturity and interrelated effects of temperatures and container on longevity of neem (Azadirachta indica) seed. The Indian Forester, 115, 89-97.
32. N. Pammenter,Patricia Berjak (1999). A review of recalcitrant seed physiology in relation to desiccation-tolerance mechanisms. Seed Science Research, 9(1), 13-37.
CrossRef DOI
33. K. Platt-Alota,W. Thomson (1987). Freeze fracture evidence for lateral phase separations in the plasmalemma of chilling-injured avocado fruit. Protoplasma, 136(2-3), 71-80.
CrossRef DOI
34. A. Ponnuswamay,R. Vinaya Rai,C. Surendran,T. Karivaratharaju (1990). Studies on maintaining seed longevity and the effect of fruit grades in neem (Azadirachta indica). Journal of Tropical Forest Science, 3, 285-290.
35. M. Rawat,R. Thapliyal (2003). Endogenous rhythm in seed germination of Dendrocalamus strictus. Seed Science and Technology, 31(1), 21-27.
CrossRef DOI
36. E. Roberts (1972). Storage Environment and the Control of Viability. Viability of Seeds, 14-58.
CrossRef DOI
37. E. Roberts (1973). Predicting the storage life of seeds. Seed Science and Technology, 1, 499-514.
38. E. Roberts,R. Ellis (1989). Water and seed survival. Annals of Botany, 63, 39-52.
39. E. Roberts,M. King,R. Ellis (1984). Recalcitrant seedstheir recognition and storage In: Holden, J.H.W. Crop Genetic Resources- Conservation and Evaluation, 38-52.
40. Y. Roederer,R. Bellefontaine (1989). Can Neem seed be expected to keep their germination capacity for several years after collection?. Forest Genetic Resources Information, 17, 30-33.
41. M. Sacande,J. Van Pijlen,C. De Vos,F. Hoekstra,R. Bino,S. Groot (1996). Intermediate storage behaviour of neem tree (Azadirachta indica) seeds from Burkino Faso. Improved methods for the handling and storage of intermediate /recalcitrant tropical forest tree seeds, 101-104.
42. M. Sacande,Folkert A (1998). A multifactorial study of conditions influencing longevity of neem (Azadirachta indica) seeds. Seed Science Research, 8(4), 473-482.
CrossRef DOI
43. M. Sacande,F. Hoekstra,K. Khoo,N. Jayanthi,F. Tsan,Krishnapillay (1998). Recalcitrant seeds. Malaysia, 1999, 12-15.
44. D. Tiwari (1992). Monograph on Neem (Azadirachta indica A. Juss).
45. P. Tompsett (1994). Capture of genetic resources by collection and storage of seeds: a physiological approach. Tropical Trees: the potential for domestication and the rebuilding of forest resources, 61-71.
46. C. Vertucci,E. Roos,J. Crane (1994). Theoretical Basis of Protocols for Seed Storage III. Optimum Moisture Contents for Pea Seeds Stored at Different Temperatures. Annals of Botany, 74(5), 531-540.
CrossRef DOI
47. Chien Wang (1982). Physiological and biochemical responses of plants to chilling stress. Horticulture Science, 17(2), 173-186.
CrossRef DOI
48. R. Willan (1985). A guide to Forest seed handling with special reference to the tropics. FAO Forestry Paper
49. Kazuki Yamawaki,Naoki Yamauchi,Kazuo Chachin,Takashi Iwata (1983). Relationship of Mitochondrial Enzyme Activity to Chilling Injury of Cucumber Fruit. Journal of the Japanese Society for Horticultural Science, 52(1), 93-98.
CrossRef DOI
50. Shizuo Yoshida,Takefumi Kawata,Matsuo Uemura,Teruo Niki (1986). Properties of Plasma Membrane Isolated from Chilling-Sensitive Etiolated Seedlings of Vigna radiata L. Plant Physiology, 80(1), 152-160.
CrossRef DOI

Download References

Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Devendra Kumar. 2016. "Storage Response of Neem (Azadirachta Indica A. Juss.) Seed under Different Moisture and Temperature Regime". Global Journal of Science Frontier Research - C: Biological Science GJSFR-C Volume 15 (GJSFR Volume 15 Issue 48 (C6)).

More Citation Formats

Select Citation Style:

Download Citation

GJSFR Volume 15 Issue 48 (C6)

Explore Journals Explore Volume Read This Issue

Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Keywords

Azadirachta indica germination percentage germination value (GV)mean germination time (MGT)viability

Classification

GJSFR-C Classification FOR Code: 270499

Submission ReceivedNovember 20, 2015
Peer Review Double Blind
Handling Editor
Accepted December 19, 2015
Published November 24, 2015

Version of record

v1.2

Issue date

November 24, 2015

Language

English

Order Article Reprint

Experience in AR

Explore published articles in an immersive Augmented Reality environment. Our platform converts research papers into interactive 3D books, allowing readers to view and interact with content using AR and VR compatible devices.

View in VR

Read in 3D

Your published article is automatically converted into a realistic 3D book. Flip through pages and read research papers in a more engaging and interactive format.

View in 3D

Article Matrices

Total Score: 101

Country: India

Subject: Global Journal of Science Frontier Research - C: Biological Science

Authors: Devendra Kumar (PhD/Dr. count: 0)

View Count (all-time): 395

Total Views (Real + Logic): 864

Total Downloads (simulated): 68

Publish Date: 2015 11, Thu

Monthly Totals (Real + Logic):

Month 1: 51 views
Month 2: 54 views
Month 3: 61 views
Month 4: 46 views
Month 5: 59 views
Month 6: 35 views
Month 7: 21 views
Month 8: 51 views
Month 9: 31 views
Month 10: 32 views
Month 11: 32 views
Month 12: 44 views
Month 13: 28 views
Month 14: 33 views
Month 15: 33 views
Month 16: 31 views
Month 17: 35 views
Month 18: 26 views
Month 19: 13 views
Month 20: 27 views
Month 21: 19 views
Month 22: 24 views
Month 23: 15 views
Month 24: 23 views
Month 25: 34 views
Month 26: 28 views
Month 27: 23 views
Month 28: 40 views
Month 29: 39 views
Month 30: 41 views
Month 31: 31 views
Month 32: 34 views
Month 33: 34 views
Month 34: 44 views
Month 35: 21 views
Month 36: 27 views
Month 37: 17 views
Month 38: 12 views
Month 39: 31 views
Month 40: 35 views
Month 41: 35 views
Month 42: 20 views
Month 43: 25 views
Month 44: 19 views
Month 45: 16 views
Month 46: 36 views
Month 47: 26 views
Month 48: 40 views
Month 49: 29 views
Month 50: 22 views
Month 51: 21 views
Month 52: 45 views
Month 53: 30 views
Month 54: 35 views
Month 55: 19 views
Month 56: 27 views
Month 57: 39 views
Month 58: 40 views
Month 59: 45 views
Month 60: 30 views
Month 61: 23 views
Month 62: 21 views
Month 63: 34 views
Month 64: 25 views
Month 65: 29 views
Month 66: 28 views
Month 67: 11 views
Month 68: 18 views
Month 69: 25 views
Month 70: 32 views
Month 71: 24 views
Month 72: 35 views
Month 73: 28 views
Month 74: 32 views
Month 75: 23 views
Month 76: 29 views
Month 77: 21 views
Month 78: 16 views
Month 79: 27 views
Month 80: 40 views
Month 81: 29 views
Month 82: 29 views
Month 83: 25 views
Month 84: 13 views
Month 85: 16 views
Month 86: 38 views
Month 87: 20 views
Month 88: 17 views
Month 89: 24 views
Month 90: 36 views
Month 91: 37 views
Month 92: 20 views
Month 93: 24 views
Month 94: 35 views
Month 95: 25 views
Month 96: 41 views
Month 97: 43 views
Month 98: 28 views
Month 99: 18 views
Month 100: 16 views
Month 101: 20 views
Month 102: 27 views
Month 103: 27 views
Month 104: 44 views
Month 105: 33 views
Month 106: 30 views
Month 107: 28 views
Month 108: 29 views
Month 109: 29 views
Month 110: 33 views
Month 111: 16 views
Month 112: 17 views
Month 113: 38 views
Month 114: 34 views
Month 115: 33 views
Month 116: 38 views
Month 117: 35 views
Month 118: 27 views
Month 119: 42 views
Month 120: 31 views
Month 121: 29 views
Month 122: 42 views
Month 123: 62 views
Month 124: 41 views
Month 125: 60 views
Month 126: 56 views
Month 127: 54 views
Month 128: 44 views
Month 129: 36 views
Month 130: 62 views
Month 131: 42 views
Month 132: 23 views

Total Views: 864

Total Downloads: 68

All Trends