Study on Enzyme Activity in the Production and Optimization of High Temperature Alkaline I-Amylase Enzyme by Bacillus Lichenoformis using Low Cost Medium Derived from Agricultural Byproducts

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M. Sathiyamoorthy
M. Sathiyamoorthy
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M.Sathiyamoorthy
M.Sathiyamoorthy
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Dr.S.Theneshkumar
Dr.S.Theneshkumar

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Study on Enzyme Activity in the Production and Optimization of High Temperature Alkaline I-Amylase Enzyme by Bacillus Lichenoformis using Low Cost Medium Derived from Agricultural Byproducts

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Abstract

Production of α-amylase enzyme by Bacillus Lichenoformis using stirred tank fermentor (BIOSTAT -E) was carried out. The strain was obtained from National Chemical Laboratory, Pune, India. Corn starch is used as the substrate. The enzyme production was studied by changing the various parameters like temperature, pH, rpm and substrate concentration. The enzyme activity shows maximum at a temperature of 350C -370C, pH 8 and 300 rpm. The maximum enzyme production was achieved, for 1% concentration of cornstarch at 35.60C and pH 9 using the fermentation medium contains yeast extract and peptone and the enzyme activity was found to be 55.93 DUN/ml.

References

11 Cites in Article
  1. A Gandhi,L Kjaergaard (1975). Effect of carbon dioxide on the formation of α‐amylase by <i>Bacillus subtilis</i> growing in continuous and batch cultures.
  2. Griffon Fogarty,A Joyce (1974). Enzymes of Bacillus species process.
  3. H Rehm,G Read (1987). a, Enzyme technology.
  4. J Jayaraman (1981). Laboratory manual in Biochemistry.
  5. James Bailey,David Ollis (1986). Biochemical engineering fundamentals.
  6. H Martha,Leonard Oseley,Keay (1970). Purification and characterization of the α -amylase of Bacillus subtillis NRRL B3411.
  7. Peter Stanbury,Allen Whitkar (1984). Principles of Fermentation technology.
  8. Pratima Bajpai,Pramod Bajpai (1989). High‐temperature alkaline α‐amylase from <i>Bacillus iicheniformis</i> TCRDC‐B13.
  9. Pratima Bajpai,Umender Sharma (1989). Production of α -amylase in a low cost medium by Bacillus lichenoformis.
  10. Seung‐hyeon Moon,Satish Parulekar (1993). A parametric study ot protease production in batch and fed‐batch cultures of <i>Bacillus firmus</i>.
  11. Yong Hee,Lower (1990). Production of alkaline protease by Bacillus lichenoformis in an aqueous two phase system.

Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

M. Sathiyamoorthy. 2014. \u201cStudy on Enzyme Activity in the Production and Optimization of High Temperature Alkaline I-Amylase Enzyme by Bacillus Lichenoformis using Low Cost Medium Derived from Agricultural Byproducts\u201d. Global Journal of Science Frontier Research - C: Biological Science GJSFR-C Volume 14 (GJSFR Volume 14 Issue C5): .

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Version of record

v1.2

Issue date

December 25, 2014

Language
en
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Production of α-amylase enzyme by Bacillus Lichenoformis using stirred tank fermentor (BIOSTAT -E) was carried out. The strain was obtained from National Chemical Laboratory, Pune, India. Corn starch is used as the substrate. The enzyme production was studied by changing the various parameters like temperature, pH, rpm and substrate concentration. The enzyme activity shows maximum at a temperature of 350C -370C, pH 8 and 300 rpm. The maximum enzyme production was achieved, for 1% concentration of cornstarch at 35.60C and pH 9 using the fermentation medium contains yeast extract and peptone and the enzyme activity was found to be 55.93 DUN/ml.

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Study on Enzyme Activity in the Production and Optimization of High Temperature Alkaline I-Amylase Enzyme by Bacillus Lichenoformis using Low Cost Medium Derived from Agricultural Byproducts

M.Sathiyamoorthy
M.Sathiyamoorthy
Dr.S.Theneshkumar
Dr.S.Theneshkumar

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