Study on Enzyme Activity in the Production and Optimization of High Temperature Alkaline I-Amylase Enzyme by Bacillus Lichenoformis using Low Cost Medium Derived from Agricultural Byproducts

M.Sathiyamoorthy

Study on Enzyme Activity in the Production and Optimization of High Temperature Alkaline I-Amylase Enzyme by Bacillus Lichenoformis using Low Cost Medium Derived from Agricultural Byproducts

M. Sathiyamoorthy * §

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§ HIMALAYAN UNIVERSITY

Study on Enzyme Activity in the Production and Optimization of High Temperature Alkaline I-Amylase Enzyme by Bacillus Lichenoformis using Low Cost Medium Derived from Agricultural Byproducts

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Abstract

Production of α-amylase enzyme by Bacillus Lichenoformis using stirred tank fermentor (BIOSTAT -E) was carried out. The strain was obtained from National Chemical Laboratory, Pune, India. Corn starch is used as the substrate. The enzyme production was studied by changing the various parameters like temperature, pH, rpm and substrate concentration. The enzyme activity shows maximum at a temperature of 350C -370C, pH 8 and 300 rpm. The maximum enzyme production was achieved, for 1% concentration of cornstarch at 35.60C and pH 9 using the fermentation medium contains yeast extract and peptone and the enzyme activity was found to be 55.93 DUN/ml.

References

11 Cites in Article

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

M.Sathiyamoorthy. 2014. "Study on Enzyme Activity in the Production and Optimization of High Temperature Alkaline I-Amylase Enzyme by Bacillus Lichenoformis using Low Cost Medium Derived from Agricultural Byproducts". Global Journal of Science Frontier Research - C: Biological Science GJSFR-C Volume 14 (GJSFR Volume 14 Issue C5).

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GJSFR Volume 14 Issue C5

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Keywords

agricultural by products alkaline enzyme bacillus lichenoformis Fermentation low cost medium α-amylase

Classification

GJSFR-C Classification FOR Code: 860399

Submission ReceivedOctober 30, 2014
Peer Review Double Blind
Handling Editor
Accepted November 24, 2014
Published December 6, 2014

Version of record

v1.2

Issue date

December 25, 2014

Language

English

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Article Matrices

Total Score: 127

Country: United Arab Emirates

Subject: Global Journal of Science Frontier Research - C: Biological Science

Authors: M.Sathiyamoorthy, Dr.S.Theneshkumar (PhD/Dr. count: 1)

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