The T-RFLP Analysis of Methanogenic Community during the an Aerobic Fermentation of Tofu Liquid Waste

1
Suranto
Suranto
2
Sunarto
Sunarto
3
Artini Pangastuti
Artini Pangastuti
4
Edwi Mahajoeno
Edwi Mahajoeno
5
Eti Setioningsih
Eti Setioningsih
1 Sebelas Maret University

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GJSFR Volume 14 Issue H4

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The increase energy demand by the due to the growth of human population could cause decreasing fossil fuel energy. In near future energy demand will increase rapidly in the future. All those problems require each country to produce and to use renewal energy. Biogas is one of the renewable energy sources that can be used as the alternative energy. This is a kind of methane gas which is formed of various organic waste biomasses such as tofu waste. The aim of this research was to study the dynamic structure of methanogens community in the anaerobic digestion process by T-RFLP method. Sample was taken from anaerobic biodigester on day: 0, 5, 10, 15, and 20 to measure the pH, temperature, CH4, and molecular analysis. The dynamic of methanogens community during anaerobic digestion was observed by the TRFLP (Terminal Restriction Fragment Length Polymorphism) technique. The restriction enzyme used in that method was Alu1. The Shannon-Wiener (H’) index was used to analyze the community diversity.

19 Cites in Articles

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Funding

No external funding was declared for this work.

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The authors declare no conflict of interest.

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No ethics committee approval was required for this article type.

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Not applicable for this article.

Suranto. 2014. \u201cThe T-RFLP Analysis of Methanogenic Community during the an Aerobic Fermentation of Tofu Liquid Waste\u201d. Global Journal of Science Frontier Research - H: Environment & Environmental geology GJSFR-H Volume 14 (GJSFR Volume 14 Issue H4): .

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GJSFR Volume 14 Issue H4
Pg. 15- 21
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Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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September 6, 2014

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English

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The increase energy demand by the due to the growth of human population could cause decreasing fossil fuel energy. In near future energy demand will increase rapidly in the future. All those problems require each country to produce and to use renewal energy. Biogas is one of the renewable energy sources that can be used as the alternative energy. This is a kind of methane gas which is formed of various organic waste biomasses such as tofu waste. The aim of this research was to study the dynamic structure of methanogens community in the anaerobic digestion process by T-RFLP method. Sample was taken from anaerobic biodigester on day: 0, 5, 10, 15, and 20 to measure the pH, temperature, CH4, and molecular analysis. The dynamic of methanogens community during anaerobic digestion was observed by the TRFLP (Terminal Restriction Fragment Length Polymorphism) technique. The restriction enzyme used in that method was Alu1. The Shannon-Wiener (H’) index was used to analyze the community diversity.

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The T-RFLP Analysis of Methanogenic Community during the an Aerobic Fermentation of Tofu Liquid Waste

Sunarto
Sunarto
Artini Pangastuti
Artini Pangastuti
Suranto
Suranto Sebelas Maret University
Edwi Mahajoeno
Edwi Mahajoeno
Eti Setioningsih
Eti Setioningsih

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