The T-RFLP Analysis of Methanogenic Community during the an Aerobic Fermentation of Tofu Liquid Waste

Suranto

The T-RFLP Analysis of Methanogenic Community during the an Aerobic Fermentation of Tofu Liquid Waste

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The T-RFLP Analysis of Methanogenic Community during the an Aerobic Fermentation of Tofu Liquid Waste

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Abstract

The increase energy demand by the due to the growth of human population could cause decreasing fossil fuel energy. In near future energy demand will increase rapidly in the future. All those problems require each country to produce and to use renewal energy. Biogas is one of the renewable energy sources that can be used as the alternative energy. This is a kind of methane gas which is formed of various organic waste biomasses such as tofu waste. The aim of this research was to study the dynamic structure of methanogens community in the anaerobic digestion process by T-RFLP method. Sample was taken from anaerobic biodigester on day: 0, 5, 10, 15, and 20 to measure the pH, temperature, CH4, and molecular analysis. The dynamic of methanogens community during anaerobic digestion was observed by the TRFLP (Terminal Restriction Fragment Length Polymorphism) technique. The restriction enzyme used in that method was Alu1. The Shannon-Wiener (H’) index was used to analyze the community diversity.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Suranto. 2014. "The T-RFLP Analysis of Methanogenic Community during the an Aerobic Fermentation of Tofu Liquid Waste". Global Journal of Science Frontier Research - H: Environment & Environmental geology GJSFR-H Volume 14 (GJSFR Volume 14 Issue 28 (H4)).

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GJSFR Volume 14 Issue 28 (H4)

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Keywords

euryarchaeota methanogenic methanosphaerula palustris terminal restriction fragment length polymorphism (TRFLP)tofu liquid waste

Classification

GJSFR-H Classification FOR Code: 960999

Submission ReceivedJune 9, 2014
Peer Review Double Blind
Handling Editor
Accepted July 2, 2014
Published July 21, 2014

Version of record

v1.2

Issue date

July 21, 2014

Language

English

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Article Matrices

Total Score: 105

Country: Indonesia

Subject: Global Journal of Science Frontier Research - H: Environment & Environmental geology

Authors: Sunarto, Artini Pangastuti, Suranto, Edwi Mahajoeno, Eti Setioningsih (PhD/Dr. count: 0)

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