Transcriptomic Profiling of Pseudomonas Putida (NBAII RPF9) Exposed to Heat and Salt Shock

Ashwitha K; Rangeshwaran, R

Transcriptomic Profiling of Pseudomonas Putida (NBAII RPF9) Exposed to Heat and Salt Shock

Ashwitha K *

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Rangeshwaran, R

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Transcriptomic Profiling of Pseudomonas Putida (NBAII RPF9) Exposed to Heat and Salt Shock

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Abstract

Pseudomonas spp. is one of the widely studied bacterium for its versatility to be used for agricultural purposes as bioagent, biostimulant, biofertilizers or biopesticides. However, the dynamics of its efficacy varied from lab to field. The advanced biocontrol technologies have enabled better understanding of adaptability of selected Pseudomonas for field applications. In our study, we subjected P.putida (NBAII RPF9) strain to heat shock and salt shock followed by microarray analysis of the gene expression under both stresses when induced independently. Few genes were expressed under both conditions whereas some of them were newly reported stress tolerance genes. The quantification of selected stress tolerance genes revealed the level of fold change of these genes under stress and its role in conferring mitigation of heat and salt shock to P.putida (NBAII RPF 9). These techniques can be considered as major criterion in selection of a potential bacterial strain for agricultural applications.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

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How to Cite This Article

Ashwitha K, Rangeshwaran, R. 2020. "Transcriptomic Profiling of Pseudomonas Putida (NBAII RPF9) Exposed to Heat and Salt Shock". Global Journal of Science Frontier Research - C: Biological Science GJSFR-C Volume 20 (GJSFR Volume 20 Issue C5).

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GJSFR Volume 20 Issue C5

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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GJSFR-C Classification FOR Code: 279999

Submission ReceivedAugust 15, 2020
Peer Review Double Blind
Handling Editor
Accepted September 13, 2020
Published September 26, 2020

Version of record

v1.2

Issue date

September 28, 2020

Language

English

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