Neural Networks and Rules-based Systems used to Find Rational and Scientific Correlations between being Here and Now with Afterlife Conditions
February 17, 2026
Neural Networks and Rules-based Systems used to Find Rational and
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Sperm sexing represents the most promising approach for production of genderspecific livestock under commercial settings. Flow cytometry is currently the method of choice for sexing sperm cells, but remains costly, has low throughput and lowers fertility in vivo. Thus, the study was aimed to test Percoll density centrifugation for sexing sperm cells in small ruminants. Semen from both rams (n = 4) and bucks (n = 4) was collected by electro-ejaculation, cryopreserved and further used for Percoll density centrifugation (PDC). Sperm sexing was confirmed by conventional PCR by determining the ratio of X and Y-bearing sperm cells. The semen before and after PDC in goats did not alter proportion of X-bearing (30%A vs 28%A; 34%A vs 41%A; 55%A vs 56%A; 42%A vs 40%A) or Y-bearing (70%A vs 72%A; 66%A vs 59%A; 45%A vs 44%A; 58%A vs 60%A).
Jose Carlos Ferreira-Silva. 2018. \u201cUse of Percoll Density Centrifugation for Sperm sexing in Small Ruminants\u201d. Global Journal of Science Frontier Research - D: Agriculture & Veterinary GJSFR-D Volume 17 (GJSFR Volume 17 Issue D6): .
Crossref Journal DOI 10.17406/GJSFR
Print ISSN 0975-5896
e-ISSN 2249-4626
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Sperm sexing represents the most promising approach for production of genderspecific livestock under commercial settings. Flow cytometry is currently the method of choice for sexing sperm cells, but remains costly, has low throughput and lowers fertility in vivo. Thus, the study was aimed to test Percoll density centrifugation for sexing sperm cells in small ruminants. Semen from both rams (n = 4) and bucks (n = 4) was collected by electro-ejaculation, cryopreserved and further used for Percoll density centrifugation (PDC). Sperm sexing was confirmed by conventional PCR by determining the ratio of X and Y-bearing sperm cells. The semen before and after PDC in goats did not alter proportion of X-bearing (30%A vs 28%A; 34%A vs 41%A; 55%A vs 56%A; 42%A vs 40%A) or Y-bearing (70%A vs 72%A; 66%A vs 59%A; 45%A vs 44%A; 58%A vs 60%A).
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