Research
Comparative Study of Lipid Profile, Oxidants, Antioxidants Levels and Thyroid Hormone Status in Normal and Abnormal Pregnancy
Aim: The aim of the study is to evaluate thyroid status, Oxidative stress & lipid profile levels in abnormal and normal pregnancies. Material and Methods: The study includes 25 abnormal pregnant women, 25 normally healthy pregnant women. Ages ranged from 20 to 30 years. Thyroid status was estimated by ELISA method. For determination of oxidative stress Malandialdehyde (MDA) is estimated as oxidants by TBARS, Uric acid is estimated by pap method, cholesterol & HDL-C by CHOD-POD method, triglycerides by GPO-POD method. Results: In the present study, we observed that TSH is significantly increased compared to normal pregnancies TSH and FT4 are slightly decreased compared to normal pregnancies. In the present study cholesterol levels were increased in abnormal pregnancies compared to normal pregnancies and decrease in HDL cholesterol were observed in preeclamptic and eclamptic pregnant women.
Comparative Study of Immunohistochemical, Hematoxylin & Eosin Staining and its Diagnostic Importance in Hirschsprungas Disease
Aim: A comparative study of immunohistochemical, hematoxylin & eosin staining and its diagnostic importance in Hirschsprung’s disease. Material and Methods: The study of 510 patients comprised colorectal, appendicectomy biopsies and myectomy specimens at various levels. The study included both ganglionic and aganglionic segments of intestine. The specimens were fixed in 10% formalin solution. In the laboratory, the sections of paraffin embedded tissues were stained H & E and compared with Cathepsin D; repeated sections were taken from these cases for the demonstration of H & E and Cathepsin D. Results: In our study of 357 cases, 223 are male children and 74 are female children (Male: Female ratio-3:1). Short segment was the most commonly occurring type constituting 229 cases (64%), while long segment was 77 cases (21.5%). The less common is the total colonic aganglionosis constituting 21 cases (5.8%). Conclusions: Cathepsin D is equally good like Acetyl cholinesterase and can be used as a reliable immune-histo chemical stain in detecting immature ganglion cells..
