Educational Journey
MSc Biochemistry • Biochemistry
Ph.D. Biochemistry (Medical Faculty) • Biochemistry
FICS
Experience
Subbaiah Institute of Medical Sciences
Professor
0 - 0 • BiochemistryVijayanagara Institute of Medical Sciences
Associate Professor
0 - 0 • BiochemistryBasaveshwara Medical College & Hospital
0 - 0 • BiochemistryEditors Role
Reviewer
GJMR
2012 -Research
Effect of Diallyl Disulphide on Protein and Lipid Glycation, and Lipid Peroxidation in Brain of Alloxan Diabetic Rats
Non enzymatic glycosylation of proteins and lipids is the main initiating factor for the pathophysiology of chronic diabetic complications. This glycation is more prevalent in insulin independent tissues like brain, kidney, RBCs, etc. Diallyl disulphide (DADS), the principle compound of garlic oil, is well known for its antihyperglycemic, antihyperlipidemic, anticarcinogenic and antibiotic properties. Hence a study was undertaken to assess the anti-glycation properties of DADS, in alloxan diabetic brain tissue, thereby to establish any usefulness of DADS in prevention of central nervous system complications in diabetes mellitus like diabetic dementia or diabetic encephalopathy. The current study showed a significant decrease (p<0.001) in glycated proteins, glycated lipids and total TBARS levels in brain tissue of DADS treated diabetic rats as compared to diabetic control rats. Hence it can be concluded that DADS helps in reducing glycation of brain proteins and lipids as well as lipid peroxidation and thus may be useful in prevention of CNS diabetic complications like diabetic encephalopathy.
Effect of Diallyl Disulphide on Renal Glycated Proteins and Plasma Sialic Acid Levels in Alloxan Diabetic Rats
Diabetes mellitus (DM) induced hyperglycation of cellular and membrane proteins may result in altered ionic nature as well as an alteration in 3 dimensional structures of these molecules, thus resulting in a possible functional variation. Sialic acid (SA), a constituent of glomerular basement membrane (GBM) is a newly established potent indicator for the development of macro and microvascular complications in DM and its elevated levels are observed in DM patients with microalbuminuria and clinical proteinuria. Probably this elevation in SA may be due to increased SA release from the renal GBM due to hyperglycation. This biochemical alteration is the main initiating factor for the pathophysiology of diabetic complication, nephropathy. Diallyl disulphide (DADS), the principle compound of garlic oil, is well known for its anti-diabetic properties. Hence a study was undertaken to assess the anti-glycation properties of DADS and its usefulness in prevention of de-sialation of GBM, in alloxan diabetic kidneys, thereby to establish any beneficial effects of DADS in prevention of renal complications in DM. The current study showed a significant decrease (p<0.001) in kidney glycated proteins and plasma SA levels in DADS treated diabetic rats as compared to diabetic control rats. Hence it can be concluded that DADS helps in preventing glycation of renal proteins and de-sialation of GBM which may be useful in prevention of diabetic nephropathy.
