Research
Establishment of Regeneration Protocol for Canola (Brassica napus L.)
The study was conducted with an aim of developing a genotype independent efficient in vitro regeneration system for Canola (Brassica napus L.) that is not grown in Bangladesh. Plant regeneration was achieved through embryogenic callus production from hypocotyls and cotyledonary leaves with petioles. The optimum medium for callus induction was found with 0.5 mgl-1 2, 4- dichlorophenoxyacetic acid (2, 4-D). The best shooting medium for canola contained 3.0 mgl-1 benzyl amino purine (BAP), 0.1 mgl-1 naphtalene acetic acid (NAA), and 5.0 mgl-1 AgNO3 and produced maximum number of shoots when kinetin was used at a concentration of 0.5 mgl- 1. The profound positive effect of AgNO3 for Canola was found for callogenesis at 0.5 mgl-1 and for regeneration at 5.0 mgl-1. In vitro regenerated shoots of canola produced well developed root system on both the media with 2.5 mgl-1 NAA and 1.0 mgl-1 indole 3-butyric acid (IBA).
An Efficient in Vitro Regeneration System for Tori (Brassica Campestris)-7
An efficient in vitro regeneration system for Tori (Brassica campestris) -7 was developed from calli of hypocotyls and cotyledonary leaves. The optimum medium for callus induction was found with 0.5 mgl-1 2, 4- dichlorophenoxyacetic acid (2,4-D). The best shooting medium contained 3.0 mgl-1 6, benzyl amino purine (BAP), 0.1 mgl-1 naphtalene acetic acid (NAA), and 5.0 mgl-1 AgNO3. Maximum number of shoots were produced when 0.5 mgl- 1 kinetin was used, whereas the combined effect of 2.0 mgl-1 BAP, 0.1 mgl-1 NAA, and 5.0 mgl-1 AgNO3 regenerated calli the most. The effect of AgNO3 was found for callogenesis at 0.5 mgl-1 and for regeneration at 5.0 mgl-1. In vitro regenerated shoots of Tori-7 developed roots on medium with 1.0 mgl-1 indole 3- butyric acid (IBA). The developed efficient in vitro regeneration protocol can be used as a baseline for Agrobacteriummediated genetic transformation of the studied plants.
