Research
A Laboratory Scale Prodution Method of Raw Manganese (II) Sulphate from Waste used Alkaline Batteries
This experimental project deals with the laboratory scale production method of raw manganese (II) sulphate from waste used alkaline batteries. The Batteries have wide range of applications which includes automobile, household, industrial, electronics, mobile gadgets etc. There are many types of Batteries like alkaline battery, zinc carbon battery, lead acid battery, nickel cadmium battery, lithium battery etc. In UAE, millions of products using the lead acid batteries and alkaline batteries. The batteries are exhausted, after many recharge cycles. These waste used batteries are disposed to atmosphere and makes many environmental pollutions. This experimental research project explains how to synthesis Manganese (II) sulphate from these waste batteries by simple method in lab scale with step by step. Because, MnSO 4 has lots of uses and can be produced from these waste batteries. It is one of the great business idea for Chemical Engineers in the present and future UAE industrial sectors.
Study on Enzyme Activity in the Production and Optimization of High Temperature Alkaline I-Amylase Enzyme by Bacillus Lichenoformis using Low Cost Medium Derived from Agricultural Byproducts
Production of α-amylase enzyme by Bacillus Lichenoformis using stirred tank fermentor (BIOSTAT – E) was carried out. The strain was obtained from National Chemical Laboratory, Pune, India. Corn starch is used as the substrate. The enzyme production was studied by changing the various parameters like temperature, pH, rpm and substrate concentration. The enzyme activity shows maximum at a temperature of 350C – 370C, pH 8 and 300 rpm. The maximum enzyme production was achieved, for 1% concentration of cornstarch at 35.60C and pH 9 using the fermentation medium contains yeast extract and peptone and the enzyme activity was found to be 55.93 DUN/ml. Since the cost of yeast extract and peptone is very high, so the further work was done using some low cost carbon and nitrogen sources like defatted cotton seed, defatted soya flour and mustard seed which are extracted from agricultural byproducts. The enzyme activity for using the low cost medium was found to be nearly triple such as 121.49 DUN/ml. The enzyme production reaches the steady phase at 24 hours. So it is highly recommended that using the low cost medium for the α-amylase enzyme gives better biomass cell concentration and enzyme activity as well.
Study on Cell Concentration in the Production and Optimization of High Temperature Alkaline I-Amylase Enzyme by Bacillus Lichenoformis using Low Cost Medium Derived from Agricultural by products
Production of α-amylase enzyme by Bacillus Lichenoformis using stirred tank fermentor (BIOSTAT – E) was carried out. The strain was obtained from National Chemical Laboratory, Pune, India. Corn starch is used as the substrate. The enzyme production was studied by changing the various parameters like temperature, pH, rpm and substrate concentration. The biomass cell concentration shows maximum at a temperature of 350C – 370C, pH 8 and 300 rpm. The maximum enzyme production was achieved, for 1% concentration of cornstarch at 350C and pH 8 using the fermentation medium contains yeast extract and peptone and the cell concentration was found to be 2.882 gm dry weight/lit. Since the cost of yeast extract and peptone is very high, so the further work was done using some low cost carbon and nitrogen sources like defatted cotton seed, defatted soya flour and mustard seed which are extracted from agricultural byproducts.
