Comparative Evaluation of Antibacterial Efficacy of Aloe Barbadensis Miller, Camellia Sinensis, Azadirachta Indica and Doxycycline with Sodium Hypochlorite as Root Canal Irrigant against E. Faecalis: An In-Vitro Study
Aims and objectives: Successful root canal treatment involves the complete elimination of pathogenic microorganisms from the root canal system. Enterococcus faecalis is one of them which is most commonly detected in asymptomatic persistent endodontic infections. Chemical irrigation of the root canals along with biomechanical preparation helps in the elimination of such microorganisms. Thus, the present study was done to evaluate and compare the antibacterial efficacy of Doxycycline, Camellia Sinensis, Aloe barbadensis Miller, Azadirachta indica and saline with Sodium hypochlorite as an intracanal irrigant against E.faecalis. Material and Method: Five different intracanal irrigants were selected for this study (ie. Doxycycline, Camellia Sinensis, Aloe barbadensis Miller, Azadirachta indica, and normal saline)and their comparison was done with Sodium hypochlorite against E.faecalisto check their antimicrobial efficacy. The extracts were prepared, and MIC was determined.A total of 6 wells per plate were made on the agar plate with the help of cork borer. Once wells on plates are made, the testing irrigatingsolutions was pipetted into each well,and then the zone of inhibition was determined after 72hrs by Agar well diffusion method. Results: The average size of zones of inhibition after 72 hours were: Doxycycline has the highest zone of inhibition (34.00 ± 0.71) followed by Azadirachta indica(16.00 ± 0.63), Camellia Sinensis(14.00± 0.71), Sodium hypochlorite (10.17 ± 0.98) (positive control), and least zone with Aloe barbadensis Miller(8.17 ± 0.75) and no zone of inhibition was observed with normal saline (negative control). Results were subjected to statistical analysis by using Mann -Whitney U test and Kruskal-Wallis test. Conclusion: Tested herbal irrigants like Aloe barbadensis Miller, Camellia Sinensis and Azadirachta indica showed inhibitory zone against E. faecalis. Hence, these can be used as root canal irrigating solutions.
