Poison in the Hiking Trail

Poison in the Hiking Trail

A. Bakarr Kanu

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Alexis Brooks

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Abstract

An approach combining reverse-phase high-performance liquid chromatography (RP-HPLC) and electrospray ionization mass spectrometry (ESI-MS) was developed to analyze Urushiol congeners in poison ivy extract. The peak signatures detected in poison ivy were separated in 18 min at wavelengths 254 nm, 260 nm, and 280 nm with a gradient elution on the RP-HPLC system. The ESI-MS data confirmed the fragmentation patterns of six Urushiol congeners (C15:0-2 and C17:1-3) detected in the poison ivy extract. Recovery studies conducted with Urushiol (15:2) show recovery within ±2%, well within the recovery efficiency of ±15-20%. The validation data showed that the limit of detection (LOD) and limit of quantitation (LOQ) for Urushiol (15:2) was 0.29 ± 0.03 ppb and 0.97 ± 0.01 ppb, respectively, with a sensitivity of 0.110 ± 0.002 mAU ppb -1 . A standard addition calibration approach was used to quantify the Urushiol (15:2) content in the poison ivy extract and reveal one poison ivy leaf may contain 0.674 ± 0.025 mg/g of Urushiol (15:2).Our investigation demonstrates the quantitation of Urushiol congeners in complex mixtures. This same approach can be beneficial for analyzing other chemical components in food and different types of complex matrices.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

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Not applicable for this article.

How to Cite This Article

A. Bakarr Kanu. 2026. \u201cPoison in the Hiking Trail\u201d. Global Journal of Science Frontier Research - B: Chemistry GJSFR-B Volume 23 (GJSFR Volume 23 Issue B1): .

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GJSFR Volume 23 Issue B1
Pg. 1- 12

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Keywords

electrospray ionization mass spectrometry poison ivy reverse-phase high-performance liquid chromatography Urushiol

Classification

GJSFR-B Classification: DDC Code: 796.5109746 LCC Code: G1241.E63

Submission ReceivedJanuary 14, 2023
Peer Review Double Blind
Handling Editor
Accepted January 21, 2023
Published February 10, 2023

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v1.2

Issue date

February 21, 2023

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