Research
Engineered Bovine Serum Albumin Nanoparticles Incorporated Alpha Amylase Preparation for the Improved Activity
In the present study, the improved enzyme activity of alpha amylase encapsulated with bovine serum albumin nanoparticle (BSA) was carried out. Initially. The method for preparation of BSA nanoparticles was optimized with various parameters such as pH, acetone to BSA ratio, cross linking time and concentration of BSA. The optimal pH was found to be 4.5, 10 mg/ml of BSA concentration, acetone to albumin ratio was to be 4.5, cross linking time of 3hrs which gives the higher yield of BSA nanoparticles. The Nano-enzyme conjugate was prepared using the optimized conditions. The encapsulation of α-Amylase Enzyme with BSA Nanoparticle was done based on hydrophobic ion pairing (HIP) complexation. And the Scanning electron micrographs of BSA nanoparticle encapsulated enzyme at the targeted size of 100nm. Distinct changes in the kinetic parameters of nano albumin encapsulated α-amylase was observed. Enzyme activity of nano encapsulated alpha amylase was found to be maximum at the pH of 8.0,temperature 80ºC and 4.5mg/ml of substrate concentration.
Anti Biofilm Effect of Biogenic Silver Nanoparticles Coated Medical Devices Against Biofilm of Clinical Isolate of Staphylococcus Aureus
Biofilm represents the most prevalent type of virulent factor of most of the pathogenic microorganism and involved in crucial development of clinical infection and exhibit resistance to antimicrobial agents. Now the biofilm is considered as major target for the pharmacological development of drugs. A biofilm serves to promote bacteria persistence by resisting antibiotic treatment and host immune responses. Antibiotics are rendered ineffective when biofilms form due to their relative impermeability, the variable physiological status of microorganisms, subpopulations of persistent strains, and variations of phenotypes present. Metal nanotechnology chemistry has the potential to prevent the formation of these life-threatening biofilms on life supporting devices. In the present study, anti biofilm effect of silver nanoparticles coated catheter against clinical isolate of Staphylococcus Aureus was studied. Silver nanoparticles synthesized by leaf extract broth of Azadirhacta indica were coated on the catheter chara-cterized by scanning electron microscopy which reveals complete dispersion of the nanoparticles on the fibre surface of the catheter and the size, shape of the particles shows uniform spherical particles with the size of 50-60 nm. Distinct effect of biofilm inhibition was recorded in the nanoparticles coated catheter and maximum inhibition was observed during 72 hour of incubation. Biochemical composition of biofilm matrix mainly total carbohydrates and total protein was highly reduced. The present study would suggests the development of anti microbial coated medical devices against pathogenic microorganism.
