Research
Comparative Studies of the Effect of Diazepam and Magnesium Sulphate on Motor Coordination and Seizures in 4-Aminopyridine Induced Swiss Mice
The comparative effect of Diazepam and magnesium sulphate on motor coordination and seizures in mice induced with 4-Aminopyridine (4-AP) was studied. Thirty six (36) Swiss white male and female mice were randomly assigned into three (3) groups of six (6) animals each for seizure and beam walking test. Group one animals served as control group and were given normal saline intreperitoneally. Group two animals were given Magnesium sulphate (M.S) (4.5mg/kg) intraperitoneally. Group three (DZP) animals receieved Diazepam (DZP) (2mg/kg) intraperitoneally for the beam walking test. While for the seizure tests, Group one (4-AP) served as control and were given 4-Aminopyridine (4-AP) (13.3mg/kg) only, intraperitoneally. Group two (M.S) animals were given 4-Aminopyridine and Magnesium sulphate (M.S) intraperitoneally 15 minutes before inducing epilepsy with 4-AP. Group three (DZP) animals received 4-Aminopyridine and Diazepam intraperitoneally 15 minutes before inducing epilepsy with 4-AP. Anti-epileptic effect was assessed by scoring the onset of both tonic and clonic seizures.
Ultravist Studies on the Histological Patterns of the Kidney of Adult Wistar Rats
The aim of this study was to assess the effects of ultravist on the histological patterns of the kidney of the Wistar rat. Thirty (30) Wister rats weighing between 182-212kg/BW were divided into three groups of ten (10) animals each. Group A served as the control group, while group B and C served as experimental groups receiving low and high dosage of ultravist (iopromide) which is the Radiographic Contrast Medium (RCM) used for the experiment respectively. Collection of tissues was carried out on rats at intervals of 30 minutes, 60 minutes, 2 hours, 12 hours and 24 hours following administration of RCM on the experimental groups. The sampling from experimental and control groups were carried out simultaneously following administration of low and high dose of RCM to rats from experimental groups. The result obtained for ultravist after one hour administration presented in plates 2 and 4 showed alteration in the high dose animals while plates 3 and 5 showed marked alterations in the cellular morphology of the kidney of the low dose animals.
