Research
Solvent Polarity and Temperature Effects on Extracted Secondary Metabolite from the Fruit of Tetrapleura Tetraptera and its Antibacterial Potential on Uropathogens
Odoriferous medicinal plants are known to be used as natural therapeutic agents, as they are rich sources of terpenoids and polyphenols. This study was aimed at evaluating the solvent polarity, temperature effects and synergistic potential of the phytochemicals in the fruit extract obtained from the fruit of Tetrapleura tetraptera on clinically isolated uropathogens. T. tetraptera has being used locally in treating some ailments. The sample was extracted using methanol, hot water and cold water respectively. The quantitative and qualitative compositional analysis of the secondary metabolites of the fruit extract was carried out using Gas chromatography-mass spectrometry (GC-MS). The antibacterial screening was carried out using agar-well diffusion assay. The GC-MS analysis of the fruit extract led to the identification of thirtyfive (35) constituents amounting to 96.28% of the extract.
Compositional Analysis, Antioxidant and Antimicrobial Potential of the Seed of Annona cinerea Dunal Grown in Nigeria
Seed of Annona cinerea grown in Nigeria was investigated for its secondary metabolites and antioxidant potential using Gas Chromatography-Mass Spectrometry (GC-MS), 2,2ʹ- dyphenyl-1-picrylhydrazyl (DPPH) and 2,6-ditert-butyl-4-[(3,5-ditert-butyl-4-λ1- oxidanylphenyl)methylidene] cyclohexa-2,5-dien-1-one (Galvinoxyl), respectively. The antibacterial activity of the seed extract was evaluated on eleven (11) pathogenic bacteria using agar well diffusion method at different concentrations of the extract. Twenty-seven (27) therapeutically active secondary metabolites were identified in the seed extract using GC-MS and the principal constituents identified were 3-O-methyl-d-glucose (52.14%), β-sitosterol (11.79%), desulphosinigrin (6.16%) and α-tocopherol (5.84%). The extract also displayed high DPPH and galvinoxyl radical scavenging activity with IC50 values of 5.0 and 100 μgml-1. The zones of inhibition ranged from 10-30 mm against all tested bacteria. The antibacterial index (AI) ranged between 0.5-25. This study demonstrated that the seed of A. cinerea could be a potential source of natural antioxidants and antimicrobial agents.
