The shipping industry is responsible for the carriage of 90% of world trade. Thus, it remains the most energy efficient mode of transport. Shipping is expected to have greater impact on global warming considering size of vessel plying the world ocean. The Green House Gas (GHG) emissions are the main air pollutants in maritime transportation. In 2007, CO₂ emission from the shipping amounted to 847 million tones or about 2.7% of global CO₂ emission and it is expected to reach 18% in 2050. In July 2009, Marine Environment Protection Committee (MEPC) approved to circulate interim guidelines on the method of calculation of Energy Efficiency Design Index (EEDI) to create stronger incentives for further improvements in ship‟s fuel consumption, resulting CO₂ emissions on a capacity basis. This paper present outcome of GHG emission data collection and quantification from ship, the study hope to contribute to regulation for reduction of GHG emission in shipping industry and subsequent mitigation of climate change. Equipment used to measure the concentration of gas and total suspended particulate in the atmosphere are Mini Vol Portable Air Sampler, Graywolf Direct Sense Monitoring Kit, TSI IAQ-Calc and Gas Detector IQ-1000. The equipment‟s are used to determine the gas concentration, nitrogen dioxide (NO₂), sulphur dioxide (SO₂) and carbon dioxide (CO₂) concentration respectively. Carbon dioxide (CO₂) is the most important anthropogenic GHG. The experimental data analysis is used to validate recommended EEDI calculation.