The influence of inorganic and organic fertigations present study. Effect of different concentrations of chemical cyanospray on seed germination at room temperature were analyzed for protein, amino acid and amylase enzyme in both untreated and treated ways respectively. Our findingsduring seed germination on A. tristis was evaluated in the indicated that during germination the amount of amylase and(CaCl2) and different organic fertilizers such as Bijamrita and amino acid increased at 48th hrs in combination with 25% Bijamrita and 0.3% cyanospray (50:50) when compared with other treatments, but, the protein content was significantly reduced during germination when compared with nongerminated seeds. Possible optimum concentration was determined to induce seed germination and thereafter growth of seedlings.