Issues: In many countries with high HIV/AIDS prevalence rates, large numbers of students, lecturers, administrators and other educational employees are becoming infected, with substantial impacts on the supply and quality of education providing access to HIV/AIDs prevention information and care is an important way of safeguarding Nigeria’s investment in higher education. Description: Since 2011, a Comprehensive HIV/AIDS Prevention Program (CHAPP) with funding from USAID is being implemented, including prevention activities, HIV/AIDs counseling and testing (HCT), antiretroviral (ARVs) and psychosocial support for students, lecturers and other educational employees in three tertiary institutions in Ekiti State Nigeria. The unique feature of this program is the recognition that tertiary institutions do not always represent safe environments particularly for women and girls. Hence, gender is a critical factor with female students/staff participating in all the program aspects. A baseline survey related to prevention and understanding Sexually Transmitted Diseases including HIV/AIDS and mobilization of stakeholders was carried out for better planning, implementation, institutional ownership, monitoring and evaluation. Lessons Learned: Participation of staff and students is crucial in achieving the stated objectives of CHAPP as demonstrated during the Peer Education Training and a high turnout of staff during the mobilization of stakeholders. As a result of these, a partnership was formed between institutional structures which encourage periodic assessment. Recommendation: Institutional leadership commitment, tertiary institutions HIV/AIDS policy, and developing a gender perspective that recognizes the greater vulnerability of women is highly recommend since they have crucial role to play in addressing the whole range of political, social, economic, legal and management implications of HIV/AIDs in higher education