This study based to assess the livestock holders’ vulnerability to climate change across four agro ecological regions of Nepal. Altogether 240 households, 60 from each agro ecological region, were selected using stratified random sampling technique. Primary data were collected through household survey and Focus Group Discussion using structured and pre tested questionnaire and analyzed by descriptive statistics. Integrated vulnerability approach based on the Principle Components Analysis to create vulnerability indices to conduct a comparative analysis of vulnerability at the regional levels. The results reveals that the farmers were aware of climate change. The result shows that livestock holders from the fragile warm temperate mountainous region are more vulnerable because of greater exposure to climatic induced hazards, catastrophe, and low adaptive capacity results from highly pauper economic condition, with limited access to basic services, assets, and poor infrastructure. Based on the results, measures to prioritize and target the vulnerable livestock holders for awareness creating activities and of off farm employment opportunities is recommended to enhance their adaptive capacity.