A 58-day feeding trial was conducted in concrete tanks (2m x 2m x 1.25m) of 400L capacity to determine the effects of O. niloticus (3.40g + 0.04) fed pelleted diet to apparent satiation at different feeding frequencies (once (FF1) at 0900hr, twice (FF2) at 0900 and 1700 h, three (FF3) at 0900, 1300 and 1700 h and four times (FF4) 0900, 1200, 1500 and 1800 hs daily respectively. Fish were fed with 35% protein diet at 5% body weight. There was a significant increase (P < 0.05) between feeding frequency of three times (FF3) daily, 19.33+ 0.67 and other feeding frequencies of once (FF1) 9.33+0.33, twice (FF2)13.67+ 0.33 and four times (FF4) daily, 17.67+0.33 with respect to final mean weight. Also Feed Conversion Ratio (FCR) of the fish fed feeding frequency of three times (FF3) daily, 13.96 + 1.66, is the best of the four feeding frequencies. O. niloticus survival was not (P < 0.05) affected by the different frequencies.