Wind tunnel is a simple but marvelous element of scientific research and expanding empire of application day by day. Wind tunnel has been used in racing cars, airplanes, weather patterns, skydiving simulations and for aerofoil testing in laboratories. In this study, a wind tunnel with isokinetic probes has been designed, fabricated and tested for sampling aerosol particles. The cross section of the wind tunnel is circular having six inch in diameter. The type of flow in the tunnel is open, uniform and the cross sectional area is constant throughout the tunnel. In this work, an atomizer generates polydisperse aerosols in a generation chamber and monodispersetest aerosols are produced by separating the polydisperse aerosols in an improved virtual impactor. The monodisperseaerosols are passed through the wind tunnel and sampling is done in the tunnel. Isokinetic sampling probes have been designed, fabricated and installed at different locations of the tunnel for sampling generatedmonodisperse aerosol particles. Probe velocity measuring device by using pressure sensor named (MPXV5050GP) is also fabricated for making the sample isokinetic. It has been found that the sampling of aerosols is better than the conventional sampling in the outlet pipe in respect of less particle loss. The monodisperse aerosols in the wind tunnel help us to carryout research on aerosol properties and to calibrate the air pollution measuring instruments available in the market.