Road safety problem draws significant attention in a developing country like Bangladesh where road accidents are extremely high. It is estimated by AAA that among eight national highways, over 14% fatal accidents occur on only Dhaka – Chittagong highway, a major transportation artery in Bangladesh. The study was intended to identify the accident- prone locations commonly termed as Hazardous Road Locations on Dhaka – Chittagong highway. The accident data were collected from the database of Accident Research Institute, Bangladesh University of Engineering & Technology, using the Microcomputer Accident Analysis Package Five (MAAP5) software. The Geographic Information System was used as a tool to identify the HRL on Dhaka – Chittagong highway. A total of 35 segments were identified as HRL on Dhaka – Chittagong Highway. This research work comprises the detail accident analysis of one segment on Dhaka – Chittagong Highway which is identified as the most hazardous among the 35 HRL.