Background: Aim of the present study was to observe the measurements of septal papillary muscles present in tricuspid valve of human heart. Measurements of septal papillary muscles in tricuspid valve gains utmost importance in cardiac surgeries because they are the causes of myocardial infarction in recent times because of its variations and detection of these causes by advent in modern technologies which will help in treatment of tricuspid valve diseases. Materials and Methods: This study was carried out on 96 normal formalin fixed human heart specimens. Dissection was performed according to standard techniques. Septal papillary muscles were observed and length, width and thickness of each muscle are measured and documented. Results: In the present study, numbers of septal papillary muscles were present with a frequency of 0-2, with most common appearance of 1 muscle in 67 hearts (69.8%) and least common incidence of 2 muscles in 6 hearts (6.3%). Septal papillary muscles were present in 73 (76%) hearts. In measurements, septal papillary muscle mean height was 0.7±0.22 cm, mean width was 0.48±0.16 cm and mean thickness was 0.34±0.12 cm respectively. Conclusion: We hope this study will serve to understand the morphometry of septal papillary muscles better and will help in various surgical procedures and cardiac treatment done on tricuspid valve.