Background: Variations in the formation of brachial plexus and in the course of its branches are not as uncommon as was once thought. Anatomical knowledge of the variations about formation, course, termination of median and musculocutaneous nerve is extremely important in surgical exploration and administration of neuromuscular blocks in axillary region. This awarenesshelpful while planning reconstructive flap surgeries and treatment of fracture of humerus. Materials and Methods: This study was carried out on 40 normal formalin fixed upper limb specimens of 20 cadavers. Dissection was performed according to standard techniques. Variation in the origin, course, branching pattern, termination and relationship between median and musculocutaneous nerve were noted and documented. Results: In the present study, out of 40 specimens absence of musculocutaneous nerve was noted in 5% of the limbs. In those 5% limbs median nerve showed variation in origin, course and termination. Conclusion: This study will serve to understand the anatomical relationship between median and musculocutaneous nerve and it will help in various surgical procedures done on upper limb.