Infective endocarditis (IE) is an infection of the endothelial surface of the heart and heart valves with serious, even fatal complications and that often requires long-term treatment. Many dental procedures may lead to IE in high-risk patients. The aim of the present study wasto assesstheawareness and knowledge of graduating medicalanddental students at Qassim University, KSA regarding prevention of infective endocarditis. A questionnaire was administered to the last year medicalanddental students. An acceptable level ofsuccessinthe test was defined as at least 7 correct answers out of 13 (53%).Out of 118 students participating in this study, 65 (47.4%) passed the test successfully. Although the pass-rate of medical students (45/93 or 48.4%) was comparatively higher than that of dental students (11/25 or 44%)the difference between the two groups was not statistically significant. This study showed that theknowledge concerning prevention of IE among the dental and medical students was moderate and highlighted the necessity of more education in this field.