A simple, sensitive, rapid, selective, precise and accurate high performance liquid chromatographic method was developed and validated for the determination of Pramipexole dihydrochloride in bulk and tablet dosage forms. HPLC separation was carried out by reversed phase chromatography on a Thermo Scientific C18 column (250 mm × 4.6 mm, 5 μm), held at ambient temperature. The mobile phase consisted of methanol: acetonitrile (40:60 v/v), run at a flow rate of 1.0 ml/min and with UV detection at 263 nm. The method was found to be linear over an analytical range of 1-100 μg/ml with LOD = 0.075 μg/ml and LOQ = 0.227 μg/ml, respectively. The proposed method was validated successfully and applied to the quantification of the drug in tablet dosage forms.