Diabetes mellitus is a group of metabolic disease characterized by a state of chronic hyperglycemia resulting from defect in insulin secretion, insulin action or both. Diabetes mellitus is a cluster of abnormal metabolic paradigm having common features of hyperglycaemia Type2 Diabetes mellitus has been shown to be a state of increased free radical activity3. Chronic hyperglycemic status favours auto-oxidation and the formation of advance glycation and products. There is a positive correlation between Adenosine deaminase and control of Type2 Diabetes Mellitus. Malondialdehyde (MDA) is the measure of lipid peroxidation of membrane lipids which is directly proportional to the oxidative stress on the membrane. The correlation of Malondialdehyde (MDA) and Adenosine deaminase(ADA) levels in relation to control of Type2 Diabetes mellitus based on HbA1C level indicate that there is an auto-oxidation of glucose which results in persistent production of Malondialdehyde (MDA) and ROS which can release advance glycation end product(AGE) and advanced lipoxidation end products ( ALE). A case control comparative study was done with Type2 Diabetes mellitus and normal controls at BMCH&RC, Chitradurga. According to the criteria, blood sample were collected under aseptic precautions and evaluation of Fasting Blood Sugar, HbA1C, Adenosine deaminase(ADA), along with RBC membrane ghost preparation and estimation of Malondialdehyde (MDA) were done. In this study, It was found that there was significant increase of Adenosine deaminase(ADA) in serum of Type2 Diabetes mellitus cases (40.06 ± 9.16) in comparison to control groups (21.21 ± 5.72) with a statistical significance of(P<0.001)along with Malondialdehyde (MDA) of RBC membrane which was also significantly increased (4.23 ± 0.21) in Type2 Diabetes mellitus in comparison to normal control (3.28 ± 0.19) with a statistical significance of P<0.001. In our study, the positive correlation of membrane Malondialdehyde (MDA) and serum Adeno