Background: Pseudomonas aeruginosa is emerging as a nosocomial pathogen by producing Metallo Beta lactamases and acquiring resistance to many antimicrobial agents. Materials and methods: 132 isolates of Pseudomonas aeruginosa from various clinical samples were tested for MBL production by double disk synergy method. Antibiotic susceptibility pattern was done by comparing non-MBL producers and MBL producers. Results: Pseudomonas aeruginosa was isolated from Pus-39.39%, from urine-37.87%, sputum-3.03%. MBL producers were 44(33.34%), non MBL producers were 88(66.6). all MBL producers were 0% sensitive to Imipenem and Meropenem.