Microflora (fungal and algal flora) inhabitants different categories of macrohydrophytes namely; floating submersed, emmersed and ferns were isolated and collected from fresh water courses (Nile branches, channels and drains) at middle Egypt belt surveyed for their degrees of attachments to hydrophytes and mainly classified into loosely, moderately and firmly attached microflora. Occurrence range of isolated organisms, their total counts from phylloplane were investigated. Ecological analysis (physical and chemical) of water from which samples collected were done. Also preliminary study of the enzymatic activities of fungi isolated from different hydrophytes was examined pectinase, cellulose, lipase and amylase. There is no clear relationship between the degree of attachment and the enzymatic of the fungal flora of phylloplane isolated.