The current study was designed to investigate the changes of liver enzymes during normal pregnancy. To achieve the intended aim, 185 pregnant women of aged 20 – 37 years(60 women in first trimester, 65 women in second trimester and 60 women in third trimester of pregnancy), also the study contain70 women(control individuals) in age near to age of pregnant women. The levels of Alanine amino transferase(ALT), Aspartate amino transferase(AST) and Alkaline Phosphatase(ALP) were determined by enzymatic methods. The results indicated a significant (P<0.05) increase of ALT and significant(P<0.01) increase of AST activities in pregnant women in third trimester when compared with those of the control group, while ALP indicated higher significantly(P<0.0005) in third and second trimester when compared with control group.