The present research was aimed to study the effect of different levels of inorganic fertilizer N on the yield and yield components of barley varieties at Gorgan Research Station, Iran in 2014-2015 year. A split plot layout within randomized complete block design with 3 replications was used. Main plot were different level of nitrogen fertilizer (35,70 and 105 kg N ha-1) from urea source, and sub plot were different varieties (Line 3, Line 7 and Line 17). Condition represented the effect of nitrogen was significant on feed and grain yield, Protein yield, Plant height, HI. Maximum Plant height, HI and grain yield was recorded in sterling. The highest Feed yield, grain yield was observed in Line 5 variety. Nitrogen applied at the rate of 105 kg N ha-1 resulted in maximum Plant height, Harvest Index, feed yield, grain yield, Protein yield.