Genetic evaluation was estimated for salt tolerance between the selected wheat genotype through plant breeding program and local cultivar by using random amplified polymorphic DNA (RAPD-PCR reaction) with two selected primers (OP1-01 and GB8). According to the amplification results, the genetic distance, Dendrogram illustrated genetic fingerprint and relationships between the selected genotype (Dijila) and local cultivar (Tamooze-2) were determent under non and saline condition. Also the chlorophyll content and organic compounds of the upper leaves were measured.