A diazinum salt of Benzidine was coupled with aniline to form 4, 4-Bis (1"-azo-4"- aminobenzene) biphenyl. The resulted azo dye was polymerized with different diacid chloride to form azopolymers. Monomer azodye and its azopolymer were determined using elemental analysis , Infrared spectroscopy and HNMR. The microbial degradation constant and rat of azopolymer were quantitatively assessed against fungi ( Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus ochraceous and Fusarium oxysporum ). Pleurotus ostreatus Hngary (HAR17) And Ganoderma resencium mycelial hyphae, Candida albicans was used as yeast model. However, Escherichia coli was used as bacterial organisms, using different concentration from azopolymers.