The shaft of the thresher must be stiffness and strength to thresh efficiently for long duration. The objective of this study is to carry out the dynamic stiffness analysis of the shaft for thresher and performance of the thresher. The threshing scheme is used to change the operating speeds and moisture content of the paddy field (grain). Dynamic stiffness and performance were analyzed by using Hooke’s law. To enhance the threshing efficiency between dynamic stiffness of the shaft for thresher and losses as an unbalance weight was attached on the shaft. The analysis can be used for un-threshed losses and total losses. Performance due to dynamic stiffness was developed based on experimental performance. The most total grain losses of 12.263% were recorded at threshing thresher speed of 5.31 m/s at experiment. At 17% moisture content, un-threshed grain is 2.01% and threshing capacity is 97.99%.