Bacterial blood stream infections can lead to life threatening sepsis that requires rapid antimicrobial treatment otherwise may lead to morbidity and mortality of patients. Blood culture is gold standard technique which provides essential information for the diagnosis and appropriate medication to save life of affected patients. Present study was conducted to determine the bacteriological profile of blood stream infections and their antibiotic susceptibility pattern in patients visiting Janamaitri Hospital, Balaju, Kathmandu, Nepal. A total of 838 blood samples were collected from the clinically suspected cases of bacteremia and septicaemia. Isolates were identified by standard biochemical tests, and antibiotic susceptibility test was performed by using CLSI guidelines. Positive blood culture was obtained in 61/838 (7.28%) where gram negative accounted for 48/61 (78.69%) in which Salmonella Paratyphi A was leading organisms and gram positive accounted for 13/61(21.31%) in which Staphylococcus aureus was leading organisms.