Accumulation of heavy metals in crops grown in metal-polluted soil may easily cause damage effect on human health through food chain. Fu et al (2008), The main factor for heavy metals accumulation was litho logical factor in three acid agricultural areas. It is reported that soil (aquaregia) soluble fraction of Co, Ni , Al and Fe. These elements were associated with indigenous clay minerals in the soil high in toxic metals. Species Helianthus Annuus (Sunflower) grown in contaminated soil and after washing with tap water. The soil were mixed with different amendments (SSP, CaCo3 , FYM and CaCo3+ FYM).After harvesting the plants were treated with Di–Acid solution and prepare sample with double distal water. The observed uptake of concentration of Cu (72480μg pot-1), Ni (2660 μg pot-1) and Zn(8217 μg pot-1) in sunflower species at flowering.