We report a case of a sympathetic ophthalmia that occurred after 25-gauge transconjunctival sutureless vitrectomy for a retinal detachment. Case report: A 36-year-old men suffering from high myopia underwent 25-gauge transconjunctival sutureless vitrectomy for a rhegmatogenous retinal detachment in the right eye amblyopic. Endolaser photocoagulation and C2F6 gas tamponade were used to manage several retinal holes. Three weeks after the initial surgery, he returned with a 2-day history of reduced vision and metamorphopsia in his left eye. Slit- lamp examination showed a shallow anterior chamber in the right eye and moderate anterior uveitis bilaterally. Fundus examination showed applied retina in the right eye and multifocal serous retinal detachments in the left one. A diagnosis of sympathetic ophthalmia was made and the patient was treated with intensive topical and systemic steroids. The subretinal fluid cleared in following treatment. Twelve months after the onset of inflammation, the patient’s condition was stable on a combination of oral cyclosporine and topical steroids. Sunset glow retinal changes remain, but there has been no evidence of recurrent inflammation. Conclusion: Sympathetic ophthalmia can develop after 25- gauge transconjunctival sutureless vitrectomy despite its smaller sclerotomy size. We recommend that special care should be taken to inspect for adequate closure of sclerotomy sites at the end of this operation.