The aim of this study was to investigate the prevalence and antimicrobial susceptibility of Enterobacteriaceae isolated from the saliva of high-school students from Sobral-CE, Brazil Public schools in Sobral-CE were randomly selected to participate of the investigation. Saliva samples were collected from 30 volunteers aging 15 to 19 years. The samples were inoculated into MacConkey agar, and then the microorganisms isolated were submitted to identification and antimicrobial susceptibility tests. It was found a prevalence of 23.3% of Enterobacteriaceae isolated from the saliva samples. The most common isolated microorganism was Serratia liquefaciens (31.8%), followed by Enterobacter Cloaceae (18.1%). Out of 55% of the samples showed resistance to amoxicillin with clavulanic acid. However, all the samples were sentitive to imipenem. The prevalence of Enterobacteriaceae isolated from the saliva samples was elevated, which is a concern because of the multidrug resistance character that these microorganisms presented.