Ophthalmology is an important term of medical field, which helps to visualize various diseases and treat them accordingly. Fundus images are processed so as to treat diseases like glaucoma, vein occlusions, and diabetic retinopathy (DR), obesity, glaucoma etc. There are types of supervised and unsupervised types of algorithms used so as to segment the Fundus images. There are three types of datasets available DRIVE, STARE and CHASE_DB1. These data sets are being segmented with the help of Laplace operator. This method makes preprocessing of images by using adaptive histogram equalization by CLAHE algorithm. The first step is to extract green channel and segment this image by using Laplace operator. Thus it helps to enhance extraction of blood vessels from fundus image. The detected blood vessels and measurement of these vessel is used for diagnosis of Diabetic Retinopathy (DR) and other eye diseases.