The prevalence of parasitic eggs and parasite cysts on computer mouse and keyboard in School of Science and Computer Studies, Federal Polytechnic, Ado-Ekiti, Nigeria was investigated. The total number of samples examined was one hundred and eighty (180) of which twenty nine (29) were positive. The result showed that the samples collected during the first, second, and third weeks had 13, 10, and 6 numbers of cysts and ova respectively. The highest incidence was observed during the first week. Some bacteria such as Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aureginosa and Enterococcus faecalis were also isolated from the sample with the highest incidence found in Staphylococcus aureus. It was concluded that the mouse and keyboards could be a source of disease transmission and should be disinfected appropriately and often.