Biochemicals such as Cholrophylls (a & b), Carotenoids, Protein, Vitamin C, Phenol, Niacin, Vitamin B1, Vitamin B2, Moisture analysis for 25 genotypes of Amaranthus hypochondriacus.L. has been worked out at Division of Genetics and Plant Breeding and Agrotechnology, National Botanical Research Institute, Lucknow in 2011. The analysis results with some genotypes with highly nutritious value. Out of the 25 genotypes studied, different genotypes having highest values for different biochemicals. AG 828, SKNA 211 and SKNA 21 are the best genotypes which will help in formation of products with superior nutritional quality. Thus the data emanating from the present study indicated the scope for utilizing best nutritional yielding lines for healthcare edible products and as the base material for developing nutraceuticals. The present investigation will also fill the gap regarding the processing of amaranth seeds for the development of superior quality edible food products for infants and also used in fast days. The highly nutritious lines of this crop are so high promising for supplemented nutritive food amelioration of nutritional deficiency.