The aim of this study was examine genetic diversities in bread wheat based on Agronomic and Quality Traits and SDS-PAGE Method. Bread wheat genotypes are Prostor, Eser, Daphan, Kınacı 97, Bağcı 2002, Tosunbey, Karahan 99, Katea 1, Nenehatun, Lancer, Karasu 90, Alparslan, Palandöken 97, Konya 2002 and Doğu 88 were used. Results revealed that Prostor wheat genotype differed from the other genoypes in three traits (gliadin subunits, agronomic and quality traits); Eser, Tosunbey and Kınacı 97 genotypes showed similarities in agronomic traits and gliadin subunits. Some genotypes presented similarities in each analysis and these similarities in terms of genotypes was different for each traits. Determination of genetic characteristic of genotypes in breeding programs provides genotype classification, genetic purity, similarities and dissimilarities early material selection. Gliadin pattern of wheat genotypes are free from environment, they are easy and forceful method in evaluation of genetic materials, breeding programs, pure seed productions in hexaploid wheat.