Penicillium notatum has been found years ago to be one of the leading novel candidates of fungi for the discovery of novel antibiotics. The fungus was isolated from eighty years old virgin forest Ikota, Ondo state. The fraction was obtained through column chromatography using mixture of solvents, after culturing and purification process of the extract. The fraction was characterized using gas chromatography mass spectrophotometer, the Gc-Ms results revealed Benzene, 1,2,4,5-trimethyl- having retention time 5.117,% of total 6.505%, pcymene having retention time 5.170 minutes, % of total 16.439%, Trans-Decalin, 2 methyl with retention time 5.235 minutes and % of total 9.468 %, also n-Nonadecanol-1 having retention time 5.301 minutes and 13.302 %, Naphthalene with retention time 6.161 minutes, % of total 11.369 %, 5, 8,11,14-Eicosatetraenoic acid, methyl ester, having retention time of 6.381 minutes and % of total 2.544 %. Literature reports had indicated the physiological activities of these compounds and of interest p-cymene and Naphthalene derivative to possess strong pharmaceutical uses.Penicillium notatum has been found years ago to be one of the leading novel candidates of fungi for the discovery of novel antibiotics. The fungus was isolated from eighty years old virgin forest Ikota, Ondo state. The fraction was obtained through column chromatography using mixture of solvents, after culturing and purification process of the extract. The fraction was characterized using gas chromatography mass spectrophotometer, the Gc-Ms results revealed Benzene, 1,2,4,5-trimethyl- having retention time 5.117,% of total 6.505%, pcymene having retention time 5.170 minutes, % of total 16.439%, Trans-Decalin, 2 methyl with retention time 5.235 minutes and % of total 9.468 %, also n-Nonadecanol-1 having retention time 5.301 minutes and 13.302 %, Naphthalene with retention time 6.161 minutes, % of total 11.369 %, 5, 8,11,14-Eicosatetraenoic acid, methyl ester, having retention time of